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Re: bow-tie post# 26973

Sunday, 06/05/2022 7:25:31 PM

Sunday, June 05, 2022 7:25:31 PM

Post# of 27643
Conclusions
• The EX-CELL® CD Insect Cell Medium has been formulated to optimize the growth, viability and productivity of the
Sf-rhabdovirus-negative (Sf-RVN®) Insect Cell Line. Combined, these two products form the Sf-RVN® Platform and provide
a high performant rhabdovirus-free BEVS alternative to produce recombinant proteins, Virus-Like Particles (VLP) for viral
vaccines, and Adeno-Associated Viruses (AAV) to treat genetic diseases.
• In this study we demonstrated that the EX-CELL® CD Insect Cell Medium out-performs all media tested, including the not
chemically defined media, for the growth of both Sf-RVN® and Sf9 cells.
• For the Sf-RVN® Insect Cell Line, our data shown that the EX-CELL® CD Insect Cell Medium enables the second-best production
after the EX-CELL® 420 Serum-Free Medium while improving lot-to-lot consistency of the medium, which is a common issue
with hydrolysate containing formulations.
• By comparing the productivity of the two Sf9 cells, we demonstrated that the Sf-RVN® cells had a higher SEAP productivity
than the Sf9 cells, especially when cultivated with companion EX-CELL® CD Insect Cell Medium

https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/261/022/poster-ex-cell-cd-insect-cell-medium-ms.pdf
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